Research themes of the Cardiovir team 2024-2029

1/ Viruses and sudden cardiac death (SCD).

2/Impact of viral infections in the development of cardiomyopathies (Myocarditis, CMD).

3/Study of the inflammatory response and modulation of the type I interferon response by viruses with cardiovascular tropism (EV-B).

4/Impact of the Epitranscriptome of the genome of RNA+ viruses (EV-B) on the infection of cardiovascular target cells.

Expertise and knowledge

• International expertise in infectious myocarditis, sudden cardiac death and pathophysiology of viral cardiovascular infections
• Quantitative molecular detection and genotypic identification of human viruses in endomyocardial tissue
• Cell culture of human cells (fetal and primary cardiac) (BSL2)
• Molecular biology: cloning, Crisper-Cas9, NGS sequencing approaches
• Production of modified prototype viruses by reverse genetics and viral culture
• Acute and chronic viral infection models of the cardiovascular system (use of tagged prototype viruses (GFP, Nanoluc)
• Confocal imaging, histology, immunohistochemistry on human and murine cells and tissues
• Molecular detection by biochips of viral infectious agents
• Murine models of acute and chronic cardiac infection by human enteroviruses

Structuring equipment

• Automated DNA/RNA extractors
• Proteomic approach (WB, ELISA, flow cytometry)
• Real-time-PCR systems
• NGS sequencing (S5, Ion Torent) with an automated library preparation system (IonChef)
• Imager and scanner for biochips
• Ultracentrifuge (Beckman system)
• NBS2 and NSB3 laboratories (NSB3, installation in progress)
• Secured Storage of Scientifics Data Server (SSD): storage and archiving of scientific data and results