Presentation of the team
Research themes of the Cardiovir team
- Role of 5'NC region-truncated enteroviruses in the development of the inflammatory response during acute and chronic cardiac pathologies.
- Role of human cardiotropic viruses in pathophysiological mechanisms related to sudden cardiac death in young adults.
- Study of the molecular mechanisms of cardiac viral infections by enteroviruses in immunocompetent subjects and their involvement in the development of unexplained cardiomyopathies.
- Study of the impact of viruses with cardiovascular tropism (CVB3, SARS-CoV-2) on inflammatory response and type I interferon response modulation in target cells.
- Impact of the genomic RNA epitranscriptome of cardiotropic RNA+ viruses (EV-B, SARS-CoV-2) during infection of cardiovascular target cells.
Expertise and knowledge
- International expertise in infectious myocarditis, sudden cardiac death and pathophysiology of viral cardiovascular infections
- Quantitative molecular detection and genotypic identification of human viruses in endomyocardial tissue
- Cell culture of human cells (fetal and primary cardiac) (BSL2)
- Molecular biology: cloning, Crisper-Cas9, NGS sequencing approaches
- Production of modified prototype viruses by reverse genetics and viral culture
- Acute and chronic viral infection models of the cardiovascular system (use of tagged prototype viruses (GFP, Nanoluc)
- Confocal imaging, histology, immunohistochemistry on human and murine cells and tissues
- Molecular detection by biochips of viral infectious agents
- Murine models of acute and chronic cardiac infection by human enteroviruses
Structuring equipment
- Automated DNA/RNA extractors
- Proteomic approach (WB, ELISA, flow cytometry)
- Real-time-PCR systems
- NGS sequencing (S5, Ion Torent) with an automated library preparation system (IonChef)
- Imager and scanner for biochips
- Ultracentrifuge (Beckman system)
- NBS2 and NSB3 laboratories (NSB3, installation in progress)
- Secured Storage of Scientifics Data Server (SSD): storage and archiving of scientific data and results