Expertise

The Inserm UMR-S 1250 unit is equipped with BSL-2 facilities, and rooms dedicated to molecular biology, biochemistry, and histology/immunohistochemistry/immunofluorescence. All the equipment for histological analysis of tissues and functional explorations of epithelial cell biology is also available.

CELL BIOLOGY

  • Cell lines (normal, immortalized, and tumor cells)
  • Primary airway epithelial cells (bronchial cells, bronchiolar cells, nasal polyp explants, nasal brushings)
  • Culture models (Air-liquid interface, 2D/3D migration, spheroids)

HISTOLOGY

  • Microtome and inclusion station
  • Immunohistochemistry
  • Tissue Micro-Array (TMA)

IN VIVO

  • Humanized Xenografts
  • Tumor models

ION FLOW ANALYSIS

  • Ca2+, K+, Cl- flows

The unit Inserm UMR-S 1250 has access to numerous equipment and infrastructures such as the technical platforms of URCA (Cellular and Tissue Imaging Platform/PICT, Cytometry Platform/URCACyt) and the CHU (Biology Platform in Innovative Research/PRBI).

CELLULAR AND TISSUE IMAGING PLATFORM (PICT-URCA)

Head: Pr Jean MICHEL

Wide-field microscopy
Confocal microscopy
IR and Raman spectromicroscopy
Preclinical imaging
Virtual slide imaging
Laser-capture microdissection
Electron microscopy

PICT platform

FLOW CYTOMETRY PLATFORM (URCACyt)

Head : Dr LE NAOUR

  • Cell sorting
  • Quantitative bioassay
URCACyt platform

REGIONAL PLATFORM OF INNOVATIVE BIOLOGY (PRBI)

  • PCR, typing and sequencing
  • Quantitative bioassay - Luminex system
  • Automated extraction
  • Quality control
PRBI platform